本品为来源于小鼠的抗GFAP的单克隆抗体。
宿主:小鼠
克隆性:单克隆抗体
用途:WB,FC, IHC,ICC, IF
交叉反应性:人,小鼠,大鼠
分子量:50KDa
推荐稀释比例:WB(1:1000),IF(1:100),IHC(1:100),ICC(1:100),FC(1:100)
About this Antibody | |
Name | GFAP Mouse Monoclonal Antibody |
Category | Monoclonal antibody(mAb); Primary antibody |
Isotype | IgG1 |
Purification | ProA affinity purified |
About the Immunogen | |
Immunogen | This antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to a region of GFAP. |
Gene ID | 2670 |
SwissProt | P14136 |
Synonyms | Glial fibrillary acidic protein; astrocyte |
Category | Neuroscience |
Background | GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. In addition, GFAP intermediate filaments are also present in nonmyelin-forming Schwann cells in the peripheral nervous system . |
注意事项:
1. 如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
2. 回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
3. 提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。
储存条件:-20℃,有效期一年。
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